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1、2,6-DNT对鲤鱼肝脏基因表达的影响Moreover,DNTisalsooneoftheseveralEffectsof2,6-dinitrotolueneexposureongeneexpressionintheliverofcommoncarp(Cyprinuscarpio)#XuJingbo,GaoChao*(DepartmentofEnvironmentalScienceandEngineering,NortheastNormalUniversity,Changchun130024)Abstract:Inordertocharacterizetheeffectof2,6-dinitr
2、otoluene(2,6-DNT)ongeneexpressionintheliverofcommoncarp(Cyprinuscarpio),fishwasexposedviawaterfor15daysatconcentrationsof0.195and1.95mg/12,6-DNTinsemi-staticbioassay.1.iverwasselectedastargettissue.CustommicroarrayswereconstructedfromcDNAlibrariesobtainedwithSuppressionSubtractiveHybridization-Polym
3、eraseChainenzymeactivityinliverofcommoncarpmayhaveacloserelationshipwiththegeneexpressionandstressresponsetoReaction(SSH-PCR)experiments,theexpressionofseveralgenes2,6-DNTexposureandreal-timegeneexpressionchanges.Theinvolvedinenergymetabolism,response.ThepatternofEROD,Microarraydatarevealedthatinthe
4、liverwasinfluencedbyPCRwasusedtoconfirmtheseaffectedgenesweremainlybiotransformationandstressGST,NaK+-ATPase,andGK2,6-DNT.Furthermore,theRCF,HSI,andtheavailableglycogenreservesoftheexposedfishweresignificantlylowerafter15daysofexposurethaninthecontrolfish.Throughprincipalcomponentanalysis,effectsby2
5、,6-DNT-stressineachtestgroupweredistinguished.Inthisstudy,indicationsofatrade-offbetweenthemetaboliccostoftoxicantexposureandprocessesvitaltothesurvivaloftheorganismwereseenonthemolecularlevelaswellasonhigherlevelsofbiologicalorganization.Keywords:Environmentalscience;Commoncarp;2,6-DNT;Geneexpression;Enzymeactivity;Energyreserves51015202530350IntroductionSonghuaRiver,flowingthroughJilinprovinceandHeilongjiangprovince,isoneofthesevenlargestriversinNorthChina.Overalongperiodoftime,ithasbeenseriouslypollutedby.
