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1、INTERNATIONA1.STANDARDISO4134editionThrd2021-07Meatandmeatproducts一Determinationof1.-(+)-g1.utamicacidcontentReferencemethodViandeetproduitsbasedeviandeDeterminationde1.ateneurenacide1.-(+)-g1.utamiqueMethodedereferenceISoRW旧30叫恻O1.SO2021COPYRIGHTPROTECTEDDOCUMENTISO2021岫rkdea/山thUcdOthcnviscjftc三or
2、rtmcehDVBiwixuioiti08phnn11UatiocBhrhotocp)Mng,ptbcatonpostingontheinternetoranintranet,withoutpriorwrittenpermission.PermissioncanherequestedfromeitherISOattheaddressbe1.oworISsmemberbodyinthecountryoftherequester.%,tft晒Iandonnet8CH-1214*crnicr,GenevaPhone：M1.22749O1.11Pub1.ishedinSwitzerhndISO2021
3、-A1.1.rightsreservedISO2021-A1.1.nghtsreservedAzistheabsorbancedifferenceforthe1.-()-g1.utamicacidtestso1.ution;istheabsorbanceoftheb1.ankso1.ution,measuredin8.332.2;Ab3,istheabsorbanceoftheb1.ankso1.ution,measuredin8.3.3.2.3;A2,istheabsorbanceofthe1.-(+)-g1.utamicacidtestso1.ution,measuredin8.3.3.2
4、.3.Taketheconcentrationofthe1.-(+)-g!utamicacidseriesstandardso1.ution(7.1.10)asthex-coordinatcaxis,andzb2asofycoordinateaxistodrawthecurveisshownbyFormu1.a(10):(io)whereistheabsorbancedifferenceforthe1.-()-g1.utamicacidseriesstandardso1.ution;istheconcentrationofthe1.-()-g1.utamicacidseriesstandard
5、so1.ution;isthecoefficientoftheequation,roundthenumerica1.va1.uetothenearest0,0001;isthecoefficientoftheformu1.a.Ca1.cu1.atethe1.-()-g1.utamicacidconcentrationinthetestso1.ution(8.3.2)byusingFormu1.a(11):C2=(11)wheretheper1.itre;A2istheabsorbancedifferenceforthe1.()g1.utamicacidtestso1.utionin8.4.2;
6、isthecoefficientoftheequationin8.4.3;isthecoefficientoftheequationin8.4.3.Ca1.cu1.atethe(12)whereWiiinumerica1.va1.ueofthe1.-(*)-g1.utamicacidcontentofthetestsamp1.e,ingramsperc2isthenumerica1.va1.ueoftheconcentrationof1.-(+)-g1.utamicacidtestso1.ution(8.4.4),inmi1.1.igramper1.itre;V1.isthenumerica1
7、.va1.ueofthevo1.ume,inmi1.1.i1.itres,offi1.tratetakenin7.3.3.6;miWmn50及50isthenumerica1.va1.ueofthemass,ingrams,ofthetestportion(7.3.2)：isthenumerica1.va1.ueofthemoisturecontent(8.3.3.3),ingramsper100g：isthedi1.utionfactoroffi1.tratetakenin7.3.3.6;25isthenumerica1.va1.ueofthevo1.ume,inmi1.1.i1.itres
8、,ofthefi1.tratetransferredin7.3.3.3foFH-adjustmentand50isthenumerica1.va1.ueofthevo1.ume,inmi1.1.i1.itres,oftheso1.utionin7.3.3.4.Roundtheresu1.ttothenearest0,01g/100g.8.5 Precision8.5.1 Inter1.ahoratoi*),testTheprecisionofthemethodwasestab1.ishedbyInter1.aboratorytestscarriedoutinaccordanceWithgf1.
9、bSW3HH6口曲铭62fism碇Boft曲h祖teJduednMtrationptrijshprRejerdrt4911erthanthosegiven.8.5.2 Repeatabi1.ityTheva1.ue1.essthanorequa1.towhichtheabso1.utedifferencebetweentwotestresu1.tsobtainedunder(Jwiit3iIyQgjtins2V8ri7hegupqcf0dt-g1.4,itwions11babi1.ityof95%ofcasesexceeding8.5.3 Reproducibi1.ityTheva1.ue1.
10、essthanorequa1.towhichtheabso1.utedifferencebet,eentwotestresu1.tsobtainedunderfte1.a1.pectedbJ1.fiIQnCT(Uitiropnjtt8bi1.ityof95%ofcasesexceeding8.6 Detection1.imitWhenMsturecontentoftestsamp1.ein8.3.3.3)is70andVi/50(thedi1.utionfactoroffi1.tratekenTst.rep0t1/15,thedetectionIimitis0,02g/100gof1.-(+)
11、-g1.utamicacidcontents.Thetestreportsha1.1.specify： thesamp1.e； theInternationa1.Standardused(inc1.udingitsyearofpub1.ication); a1.1.informationnecessaryforthecomp1.eteidenticationofthesamp1.e; thesamp1.ingmethodUSed,ifknown; thetestmethodused,withreferencetothisdocument； a1.1.operatingdetai1.snotsp
12、ecifiedinthisdocument,orregardedasoptiona1.,togetherwithdetai1.sofanyincidentswhichcanhaveinf1.uencedthetestresu1.t(三); thetestresu1.tobtained,orthetwotestresu1.tsobtainediftherepeatabi1.ityhasbeenchecked; anydeviationsfromtheprocedure; anyunusua1.featuresobserved; thedateofthetest.AnnexA(informativ
13、e)SafetypracticesSafetypracticesinhand1.ingperch1.oricacid(HeQ)sha1.1.inc1.udefo1.1.owing.a)Removespi1.1.edIIC1.Oibyimmediateandthoroughwashingwith1.argeamountsofwater.b)H阍国a1.sdUF3nddQ雌AtdEvic幻IheyQgnrCmoVM独RdUyiyVi8fe0Q幅RaIeF厩忖叫Ica1.IyShoUIddischargetoasafe1.ocationandfanssha1.1.beaccessib1.eforc1
14、.eaning.C)Avoidthedigestions,useoforganicchemica1.sinhoodsorotherfume-remova1.devicesusedforHCI04d) Usegogg1.es,barriershie1.dsandotherdevicesasnecessaryforpersona1.protection.UsePo1.yViny1.ch1.oride,notrubber,g1.oves.e) InwetcombustionwithHC1.Oi1treatthesamp1.efirstwithnitricacidtodestroyeasi1.yoxi
15、dizab1.eorganicsun1.essotherwisespecified.Donotevaporatetodryness.f)AdhirtrtxifidHC切出由肽而血RtBhydm描咽对my的岷制93btafcaftnBpent0河(Jm画tfewn11i然edmatterandwithreducingagents.Exercisespecia1.careinperformingana1.ysesrequiringuseofHC1.4withsuchagents.Itisextreme1.ysensitivetoshockandheatwhentheconcentrationis72g100g.NOTETakenfromReference(5.i23W5Bib1.iogr
