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1、INTERNATIONA1.STANDARDISO4768editionFirst2O23-O7Measurementmethodofanti-biofi1.mactivityonp1.asticandothernon-poroussurfacesMethodedemesurede!activityanti-biofi1.msur1.ep1.astiqueetautressurfacesnonporeuses1S0R4to8V20B1ISO2023COPYRIGHTPROTECTEDDOCUMENTISO2023IUirhM*hedbdi1.iUedotherwiseupdhi.o啪InyM1
2、.tta0DmkfifiU81.andonnet8CH-1214Vernier,GenevaPhone：M1.227490111觥ftte:丽丽BQrgPub1.ishedinSwitzer1.andContentsForewordivIntroductionv2 Scope13 Normativereferences14 Termsanddefinitions1Materia1.s14.1 Bacteria1.straintobeusedforthetests14.2 Reagents24.3 4Oj1.tureQttMkra1.andso1.ution；4132N1.1.U1.entaga
3、r24.3.3Suspensionmedium-1/5Trypticsoybroth(15TSB)24.4.4.567三fif三三rmedjum0,1%Crysta1.vio1.etaqueousso1.ution1.0%Sodiumdodecy1.su1.fateaqueousso1.utionApparatusSteri1.izationofapparatusandstorageofstockcu1.tures6.1 Dr)r-heatsteri1.ization6.2 High-pressuresteamsteri1.izationProcedure47.1 Biofi1.mformat
4、iononthesurfaceofthetestspecimens47.1.1 Pre-cu1.tureOfbacteria.47.1.3 Preparationoftestinobe1.apedinensincubatingmedium47.1.4 Inocu1.ationoftestspecimens57.1.5 Incubationoftheinocu1.atedtestspecimens57.2 7kertionfiit三ftfWens.67.2.2 Stainingoftestspecimenswithcrysta1.vio1.et67.2.3 Secondwashingoftest
5、specimens67.2.4 Biofi1.mfromspecimens7.2.5 Absorbancerecoverymeasurement77.3 Determiningtheviab1.ebacteriacount7Expressionofresu1.ts88.1 Conditionsforva1.idtest88.2 Ca1.cu1.ationofanti-biof1.mactivityonthebasisofabsorptionspectrummethod8.3 Effectivenessof8Repeatabi1.ityandreproducibi1.ity8Testreport
6、AnnexA(informative)Repeatabi1.ityandreproducibi1.ity1()Bib1.iographyForewordISO(theInternationa1.OrganizationforStandardization)isawor1.dwidefederationofnationa1.standardsttiugh(ISOtnnhnkoft11rtnihvcekdHPfff1.PbbGgbOdymtf1.CIrdrtttd6tand)GScnoWharKecf1.tdcommitteehasbeenestab1.ishedhastherighttobere
7、presentedonthatcommittee.Internationa1.organizations,governmenta1.andnon-governmenta1.,in1.iaisonwithISO,a1.sotakepartinthework.ISOco1.1.aboratesc1.ose1.ywiththee1.ectrotechnica1.standardization.Internationa1.E1.ectrotechnica1.Commission(IEC)ona1.1.mattersofTheproceduresusedtodeve1.opthisdocumentand
8、thoseintendedforitsfurthermaintenanceare蜘硼搬OfISo1.EC(1.三te翩在他网WRenee册Fhtheeditoria1.ru1.esoftheISO/IECDirectives.Part2(secwww.iso.org/direc1.ives).而图撇nts)aWKrtakestfi嗣嬲蜘帆ncte除硼，8曲删殴ntm部阚i野棍IVedaimedpatentrightsinrespectthereof.Asofthedateofpub1.icationofthisdocument,ISOhadnorreceivednoticeof（八）paten
9、t(三)whichmayberequiredtoimp1.ementthisdocument.However,imp1.ementorsare懈怖翻琳躺/mj，wWjSOQFg/眸IentS洗蝮油那ion,扁MMSPOn赖岫油嬲9的阳躲Orasuchpatentrights.Anytradeusedinthisconstitutenameendorsenent.documentisinformationgivenfortheconvenienceofusersanddoesnotForanexp1.anationofthevo1.untarynatureofstandards,themeani
10、ngofISOspecifictermsand幽幽蛔豳酒侬遮W即辆O)assEif1.眦聚inwe帼1.吼。nBar池帆四dead朝乩CSvbu11uisobrgiso/foreword.htmI.Anyfeedbackorquestionsonthisdocumentshou1.dbedirectedtotheuser,snationa1.standardsbody.Acomp1.ete1.istingofthesebodiescanbefoundatwww4skeFgf1.embeFfUHmkIntroductionenvironmentmicroorganismssuchthes1.im
11、es.microorganisms,andarewide1.yfoundinour1.iving“invisib1.eantivira1.testsurfaceofnon-porouseva1.uatingSimi1.ariyrsameporousantibacteria1.testandthenon-poroussurfaces.-A1.1.rightsreservedBiofi1.msareknowntobecompositionsofsubstancessuchaspo1.ysaccharides,Iipidsrandnuc1.eicacidsproducedbyvisib1.eharm
12、sandasce1.1.sofu,hercwaterpresent.ISO22196onantibacteria1.testandISO21702onantivira1.testarethetestmethodsforeva1.uatingISO18184onhygiene,*onthethetestmethodsfoqiroducts.theISOof20743onproducts.Thisdocumentdescribeshowtoeva1.uateproductsthatarctreatedtosuppressbiofi1.rnformationsonMeasurementmethodo
13、fanti-biofi1.mactivityonp1.asticandothernon-poroussurfaces1 ScopeThisdocumentspecifiesatestmethodtoeva1.uatetheanti-biofi1.mactivityofanti-biofi1.mtreatedt。PjJnt由Kan6由CihwHWiortjCffiCunfingsrtiptwiMfceraxdH)gstainteeiJi)rtdjIrtapd11UhhrI(Ix1.jJp1.anb1.ePhotocata1.yticmateria1.sareoutofitsscope.括ct祈需
14、丽超牌WftGct商匐椭I愉羔拌徜挣M魏晓隰就曜利晶氟4ectedviHetstaining-Absorbancemeasurementassayisusedtoquantifytheamountofbiofi1.mformationinthisdocument2 NormativereferencesTherearenonormativereferencesinthisdocument.3 TermsanddefinitionsForthepurposesofthisdocument,thefo1.1.owingtermsanddefinitionsapp1.y.ISOandIECmaintaintermino1.ogydatabasesforuseinstandardizationatthefo1.1.owingaddresses：ISOOn1.inebrowsingp1.atform:avai1.ab1.eathttps:/www.iso.org/obpX1.IECE1.cc1.ropcdia:avai1.ab1.eath1.www.e1.eeroped.Fbiofi1.mmicrobia1.ce1.1.sandtheirmetabo1.itessuc
