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1、1穴位肥大细胞及其颗粒物在针刺镇痛中的作用针灸经络研究回顾与展望国际学术研讨会针灸经络研究回顾与展望国际学术研讨会2Outline&穴位肥大细胞在针刺镇痛效应中的作用&穴位胶原纤维在针刺镇痛效应中的作用&穴位注射组胺的镇痛效应&手针和电针穴位信号的启动机制THE JOURNAL OF SCIENCE&HEALING,2008THE JOURNAL OF SCIENCE&HEALING,2008，4 4（3 3），），170-177170-177Animal modelAnimal model ：AA AA (acute arthritis)(acute arthritis)RatRatComp
2、lete Freunds adjuvant Acupoint selectedAcupoint selected：Zushanli Zushanli (ST36ST36)Treat effectTreat effect：Ease painEase pain，Pain Pain thresholdthresholdIDGroup Name组别组别Average SEM(%)AControl正常对照组正常对照组1.90.1B1Acupuncture at ST36穴位针刺组穴位针刺组11.60.6*B2Acupuncture at Nearby-ST36旁开针刺组旁开针刺组8.90.3*C1Inj
3、ection of DSCG at ST36色甘酸钠注射组色甘酸钠注射组1.70.1*C2Injection of NaCl at ST36生理盐水注射组生理盐水注射组1.40.1*D1Acupuncture after DSCG at ST36色甘酸钠注射色甘酸钠注射针刺组针刺组6.20.4*D2Acupuncture after NaCl at ST36生理盐水注射生理盐水注射针刺组针刺组8.90.4*D3Acupuncture opposite side after DSCG色甘酸钠注射色甘酸钠注射对侧针刺组对侧针刺组9.30.1*各组别大鼠痛阈变化率峰值比较（各组别大鼠痛阈变化率峰值比
4、较（2020分钟时）分钟时）(n=6)(n=6)Note:*P0.05 vs.Group A;P0.01,P0.01,P0.05 vs.Group B1;P0.05 vs.Group B1;P0.01,P0.01,P0.05 vs.Group D1P0.05 vs.Group D1Group Name组别组别MC Degranulation Ratio(Average SEM)()SkinMuscleControl正常对照组正常对照组29.21.226.31.8Acupuncture穴位针刺组穴位针刺组48.93.9*32.23.1*Acupuncture after DSCG色甘酸钠注射针刺
5、组色甘酸钠注射针刺组33.72.828.52.3Acupuncture after NaCl生理盐水注射针刺组生理盐水注射针刺组50.42.5*29.72.3穴区组织肥大细胞脱颗粒率统计穴区组织肥大细胞脱颗粒率统计 (n=6)(n=6)Note:*P0.01,*P0.05 vs.Control Group;P0.01 vs.Acupuncture Group.P0.01 vs.Acupuncture Group.Degranulationof cellsTHE JOURNAL OF SCIENCE&HEALING,2008THE JOURNAL OF SCIENCE&HEALING,2008，
6、4 4（3 3），），170-177170-177lThe acupuncture effect is positive relationship to the degranulation of mast cell at acupoint.lWhat is key factor conduce the degranulation of mast cell during acupuncture manipulation?lOur focus is on the collagen at acupoint.2023-11-3013To observe the function of collagen
7、 fibers in MA analgesia.7 Animal groups setting:normal group(N):Normal healthy animal control group(M):Animal with AA injection of collagenase at Zusanli(ST36)group(C)manual acupuncture group with rotation manipulation(R)or lift-thrust manipulation(L),rotation group with collagenase pretreatment to
8、destroy Zusanli(ST36)collagen fibers(CR),lift-thrust group with collagenase pretreatment to destroy Zusanli(ST36)collagen fibers(CL),lift-thrust group pretreated with collagenase to destroy Dubi(ST35)collagen fibers(CDL)(R)(L)2023-11-3014Analgesia effects observed in different groupsGroupnPain Thres
9、hold(PT)4d Before MA(s)After MA(s)Change Rate(%)MC Degranulation Ratio(%)N89.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.94.7R85.440.37*7.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85.270.62*5.390.573.310.42.4CL86.050.43*6.260.793.812.42.3注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处
10、理前痛阈：P0.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插组:P0.05 P0.05。2023-11-3015Analgesia effects observed in different groupsGroupnPain Threshold(PT)4d Before MA(s)After MA(s)Change Rate(%)MC Degranulation Ratio(%)N89
11、.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.94.7R85.440.37*7.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85.270.62*5.390.573.310.42.4CL86.050.43*6.260.793.812.42.3注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处理前痛阈：P0.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较捻转组(L)(L)痛阈变化率：痛阈
12、变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插组:P0.05 P0.05。2023-11-3016Analgesia effects observed in different groupsGroupnPain Threshold(PT)4d Before MA(s)After MA(s)Change Rate(%)MC Degranulation Ratio(%)N89.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.94.7R85.440.37*7
13、.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85.270.62*5.390.573.310.42.4CL86.050.43*6.260.793.812.42.3注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处理前痛阈：P0.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插组:P0.05 P0.05。co
14、llagen fibers of skin and muscleMast cells in skinGroupNormalGroupInjectControlcollagen fibers of skin and muscleMast cells in skinConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120Acupunc.whirling Acupunc.lift and thrust collagen fibers of skin and muscleMast c
15、ells in skincollagen fibers of skin and muscleMast cells in skinConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120 Mast cells degranulation before and after collagen destroyed Collagen destroyedCollagen destroyedConnective Tissue Research,2008Connective Tissue R
16、esearch,2008，5050（2 2）:110-120:110-120collagen fibers and mast cells in normal acupointcollagen fibers enlacement and mast cells degranulation while whirlingcollagen fibers tightened and mast cells degranulated while lifting and thrustingcollagen fibers were destroyed and mast cells had no degranulationConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-1202023-11-3021The mast cells in acupoint may translate the mechanical signal into the biological information o
